Device

Part:BBa_I766671:Design

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)


YopH-EE under weak constitutive promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 345
    Illegal PstI site found at 1223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1223
    Illegal NotI site found at 1782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1765
    Illegal XhoI site found at 863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 345
    Illegal PstI site found at 1223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 345
    Illegal PstI site found at 1223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.



Source

conposite part

References